Konferenzen zum Thema Genomforschung und Bioinformatik in Deutschland

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1
EMBL Course: RNA Sequencing Library Preparation - How Low Can You Go?
19. Mär 2018 - 23. Mär 2018 • Heidelberg, Deutschland
Zusammenfassung:
Aim of this course is to prepare RNA libraries for Illumina sequencing by using the novel NEBNext Ultra II Directional RNA kit for low input applications. Throughout the course, we will be generating libraries from ultra- low as well as from normal amounts of RNA (1 ng to 50 ng) input to better understand the impact of input amounts and PCR cycles on the bioinformatics dealing with complexity, coverage, duplication rates and uniformity of libraries. The course participants will be guided with tips and tricks through the work flow. Finally, the bioinformatics analysis of the data will be discussed. Selected participants who – PRIOR to the course - will be able to prove their samples have required quality according to provided specifications will have the chance to bring maximum two of their own eukaryotic total RNA samples to process. At least 100ng of sample will be required for attendee samples.
Eintrags-ID:
956945
Verwandte Fachgebiete:
2
EMBL Course: Next Generation Sequencing: Whole Genome Sequencing Library Preparation
16. Apr 2018 - 20. Apr 2018 • Heidelberg, Deutschland
Zusammenfassung:
The goal of this course is to teach the fundamentals of NGS library preparation and validation using enrichment based tests like the Nextera / TruSight Rapid Capture assay. We will start with an introduction into Illumina NGS technology and the library preparation workflow. The main part will be the wet lab preparation of libraries from genomic DNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quality control and quantify the prepared libraries. We will also have a basic training on assay design using DesignStudio and data analysis of Enrichment based sequencing results using standard Illumina software tools like MiSeq Reporter and VariantStudio.
Eintrags-ID:
956281
Verwandte Fachgebiete:
3
EMBL Course: Next Generation Sequencing: RNA Sequencing Library Preparation
23. Apr 2018 - 27. Apr 2018 • Heidelberg, Deutschland
Zusammenfassung:
The goal of this course is to teach the fundamentals of NGS library preparation and validation using enrichment based tests like the Nextera / TruSight Rapid Capture assay. We will start with an introduction into Illumina NGS technology and the library preparation workflow. The main part will be the wet lab preparation of libraries from genomic DNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quality control and quantify the prepared libraries. We will also have a basic training on assay design using DesignStudio and data analysis of Enrichment based sequencing results using standard Illumina software tools like MiSeq Reporter and VariantStudio.
Eintrags-ID:
956429
Verwandte Fachgebiete:
4
EMBO Practical Course — Microbial metagenomics: A 360º approach
23. Apr 2018 - 30. Apr 2018 • Heidelberg, Deutschland
Themen:
Microbiology, Virology & Pathogens | Genomic & Computational Biology
Eintrags-ID:
916499
Verwandte Fachgebiete:
5
EMBL Course: Genome Engineering: CRISPR / Cas
17. Sep 2018 - 21. Sep 2018 • Heidelberg, Deutschland
Zusammenfassung:
This course will provide hands-on training in genome editing and cell engineering in mammalian cells using the highly efficient CRISPR/Cas9 system. Participants will learn design of CRISPR targets using bioinformatics tools, generation of gene knockouts/knock-ins, and target validation using the most current technologies. This course is aimed at researchers who are familiar with basic molecular and cell biology techniques and want to learn how to create an engineered mammalian cell lines using the most recent and advanced CRISPR/Cas9 system. No previous experience in genome editing is required.
Eintrags-ID:
956872
Verwandte Fachgebiete:
6
EMBL Course: Next Generation Sequencing: RNA Sequencing Library Preparation
24. Sep 2018 - 28. Sep 2018 • Heidelberg, Deutschland
Zusammenfassung:
The goal of this course is to teach the fundamentals of NGS library preparation and validation using the Illumina TruSeq stranded total RNA sample preparation kit. We will start with an introduction into Illumina NGS technology and the library preparation workflow. The main part will be the preparation of libraries from total RNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quantify and quality control the prepared libraries, before discussing solutions for total RNA sequencing data analysis.
Eintrags-ID:
956937
Verwandte Fachgebiete:
7
EMBO | EMBL Symposium: Systems Genetics: From Genotypes to Molecular Phenotypes to Complex Traits
24. Okt 2018 - 27. Okt 2018 • Heidelberg, Deutschland
Zusammenfassung:
Systems genetics is the extension of genetics to the system-level. It aims at understanding the relationship between genotypes and molecular phenotpes at the genome-scale, thus integrating genomics with large-scale molecular and phenotypic profiling. This opens the door to the investigating of the genetic basis of complex traits in a variety of model systems, from yeast growth phenotype to human disease. Taking advantage of natural genetic variation allows to investigate the effect of many mild genetic perturbations, an approach which contrasts with the all-or-none targeted genetic perturbations.
Eintrags-ID:
956975
Verwandte Fachgebiete:

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Stand vom 15. Oktober 2017