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Lecture and Laboratory Topics
Cells of the adaptive immune system and of the innate immune system; special focus on B lymphocytes and T lymphocytes; Interactions between antibodies and antigens; Genes encoding antibodies; Immunological memory and the mechanisms involved in recall responses; The Major Histocompatibility complex (MHC) and how it controls adaptive immune response; Immunological tolerance and how the immune system is kept under control; Immunotherapy; How to generate and produce monoclonal antibodies; Using a flow cytometer for the analysis of cells of the immune system; Immunological assays (T cell proliferation, cytotoxic T lymphocyte cell assays, Western Blotting/Immunostaining, ELISA); The Mucosal Immune System.
The program will include networking and workshops designed to outfit participating startups with tools for success in partnering, networking, operations and attracting funding. There will also be a startup pitch competition held in front of a judging panel, providing a platform for assessment of the quality of pitches and feedback on improving effectiveness. Applications to participate in the pitch competition can be submitted by academic technology transfer or commercialization groups presenting their best technology for licensing, as well as startup companies created in the past two years.
Objectives: This hands on training will equip the participants with experience, knowledge and skill sets necessary to analyze and understand different NGS data.
Highlight: Participants will work with a Graphic User Interface based Linux Desktop environment in the Amazon Cloud, that is specially configured to run popular open source NGS analysis tools. Participants will be able to save and take home a copy of the fully configured Amazon Machine Image for their personal use after the training.
Hands on Skills/Tools taught: ● Data quality analysis - FastQC ● Trimming - BBDuk ● Mapping - Tophat, BBMap, bowtie ● Alignment analysis- SAM Tools ● Visualization - IGV ● Differential expression - CuffDiff ● Variant calling - GATK,SAM Tools, BCFtools, FreeBayes, LUMPY ● Filtering and processing - BEDTools, VCFtools, PICARD ● Peak calling - MACS2 ● Data Integration and analysis - Cytoscape ● Workflows - Galaxy ● Data management- Amazon Web Services, Cloud computing, Linux
Over two days, the Next Generation Sequencing USA Congress will look at key therapeutic applications, the diagnostic and clinical potential of NGS technologies, and big data and bioinformatics challenges and solutions. Our panel of expert speakers will offer insights into their latest research into areas ranging from the use of NGS in cancer and genomics to cloud computing, data storage and personalised medicine.
Over 250 delegates representing internationally renowned research & academic institutions, clinical and research institutions, as well as leading pharmaceutical and biotech companies
Over 60 presentations and case studies focused on novel next generation sequencing platforms & technologies, the applications of NGS & key developments in cancer drug development and clinical diagnostics
Including 6 interactive streams:
2nd Annual Next Generation Sequencing USA Congress
- Advances in NGS Platforms and Key Therapeutic Applications
- NGS Data Management and Bioinformatics
- NGS Clinical Applications & Diagnostics
2nd Annual Single Cell Analysis USA Congress
- Single Cell Analysis and Transcriptomics
- Single Cell Analysis Technologies
- Overcoming Key Single Cell Analysis Challenges
14 pre-scheduled one to one meetings, exhibition and informal networking opportunities.
The co-located Single Cell Analysis USA Congress will focus on single cell genomics and transcriptomics, novel techniques in imaging and cytometry as well as implications for drug development and clinical development in immunology, oncology and other therapeutic areas.
Last updated: 19 August 2016