The discovery and study of the major developmental signaling pathways (Notch, Hh, Wnt, TGF-beta, Hippo, RTK) have illuminated how patterning and growth are controlled in metazoans. These pathways are reiteratively used during development and adult life, acting to maintain stem cells and direct tissue repair and regeneration. When they are disrupted, cancer develops. This conference embraces recent progress in quantitative biology, mathematical modelling, single cell analysis and intravital imaging. It also presents a major opportunity to bring together knowledge from developmental systems with adult homeostasis, regeneration and cancer, as well as to elucidate how different developmental signals are integrated to influence cellular decision-making processes. Bringing together cell/developmental biologists and stem cell, regeneration and cancer specialists, with a strong emphasis on multidisciplinary approaches that incorporate systems biology, mathematics and physics into the study of living systems, the conference aims to: 1) Explore the commonalities between signaling in development, regeneration and homeostasis; 2) Leverage quantitative, single cell and systems biology to study the dynamics and integration of developmental signaling across scales; and 3) Highlight how perturbations in developmental pathways cause diseases such as cancer. Pairing the conference with the symposium on “Cellular Plasticity: Reprogramming, Regeneration, and Metaplasia” will also enhance the conferences’ impact and enable in-depth exploration of the parallels between plasticity/pluripotency in development, regeneration and disease.